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Advanced NMR Techniques for Glycan-Protein Interaction Analysis

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¹⁵N-Heteronuclear Single Quantum Coherence (HSQC) Titration Experiments to Monitor the Chemical Shifts of the ¹H and ¹⁵N NMR Resonances

JoVE Journal
Chimica

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JoVE Journal Chimica

¹⁵N-Heteronuclear Single Quantum Coherence (HSQC) Titration Experiments to Monitor the Chemical Shifts of the ¹H and ¹⁵N NMR Resonances

¹⁵N-Heteronuclear Single Quantum Coherence (HSQC) Titration Experiments to Monitor the Chemical Shifts of the ¹H and ¹⁵N NMR Resonances

Advanced NMR Techniques for Glycan-Protein Interaction Analysis

DOI:

10.3791/201627-v

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02:00 min

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May 17, 2024

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Sara Bertuzzi, Ana Poveda, Ana Ardá², Ana Gimeno², Jesús Jiménez-Barbero^2,3

¹CIC bioGUNE, Basque Research and Technology Alliance (BRTA), ²lkerbasque, Basque Foundation for Science, ³Centro de Investigacion Biomedica En Red de Enfermedades Respiratorias

Tags

Glycan-protein Interactions Nuclear Magnetic Resonance (NMR)Saturation Transfer Difference (STD)Heteronuclear Single Quantum Coherence (HSQC)Molecular Recognition Chemical Glycobiology

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Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Acquisition, Processing, and Analysis of ¹H-Saturation Transfer Difference (STD) NMR to Characterize Protein-Glycan Interactions

¹⁵N-Heteronuclear Single Quantum Coherence (HSQC) Titration Experiments to Monitor the Chemical Shifts of the ¹H and ¹⁵N NMR Resonances

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