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Immunologia e infezioni

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Procedura di citometria a flusso per misurare il pH relativo del glicosoma in Trypanosoma brucei vivente

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living Trypanosoma brucei

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Procedura di citometria a flusso per misurare il pH relativo del glicosoma in Trypanosoma brucei vivente

18 Views

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02:15 min

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January 19, 2024

DOI:

10.3791/201369-v

DOI:

10.3791/201369-v

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02:15 min

January 19, 2024

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1 Views

Daniel Call*¹, Sabrina S. Pizarro*^2,3, Erica Tovey, Emily Knight³, Carrie Baumgardner⁴, Kenneth A. Christensen, James C. Morris

¹Department Chemistry and Biochemistry,Brigham Young University, ²Eukaryotic Pathogens Innovation Center,Clemson University, ³Department of Genetics and Biochemistry,Clemson University, ⁴Department of Physics and Astronomy,Clemson University

Tags

Flow Cytometry Glycosome PH Trypanosoma Brucei PHluorin Propidium Iodide Thiazole Red Forward Scatter Side Scatter Relative Fluorescence Intensity

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