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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing
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Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:
10.3791/200860-v

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02:37 min

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November 10, 2023

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, , , , , ,
1Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, 2Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, 3Department of Hematology,Erasmus Medical Center, 4Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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GutZebrafish LarvaeFACSCell IsolationDissociationPapainAgarose PlateHEPESE3 MediumDissectionIntestineCell StrainerCentrifugationDAPICell Labeling

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