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MISSION esiRNA for RNAi Screening in Mammalian Cells
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May 12, 2010
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Here we use a human esiRNA library in a high-throughput screen for genes involved in cell division. We demonstrate how to set up and conduct an esiRNA screens, as well as how to analyze and validate the results.
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MISSION EsiRNARNAi ScreeningMammalian CellsGene Expression ControlSmall Interfering RNAs (siRNAs)DicerRNA-induced Silencing Complex (RISC)Target MRNA CleavageLoss Of Gene Function StudiesChemical Synthesis Of SiRNAsEndonucleolytic CleavageTarget-specific Long Double-stranded RNAsRNase IIIEndoribonuclease-prepared SiRNAEfficacy Of SiRNAs And EsiRNAsOff-target Effects
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Theis, M., Buchholz, F. MISSION esiRNA for RNAi Screening in Mammalian Cells. J. Vis. Exp. (39), e2008, doi:10.3791/2008 (2010).
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