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在哺乳动物细胞的RNAi筛选使命esiRNA

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

MISSION esiRNA for RNAi Screening in Mammalian Cells

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在哺乳动物细胞的RNAi筛选使命esiRNA

15,907 Views

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15:31 min

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May 12, 2010

DOI:

DOI:

•

15:31 min

May 12, 2010

•

6 Views

Mirko Theis¹, Frank Buchholz¹

¹Max Planck Institute of Molecular Cell Biology and Genetics

Summary

这里我们使用一个参与细胞分裂的基因的高通量筛选人类esiRNA库。我们演示了如何设置和行为的一个esiRNA屏幕，以及如何分析和验证的结果。

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