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Immunologia e infezioni

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Coloration par immunofluorescence des pièges extracellulaires des neutrophiles dans des échantillons de tissus

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

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Coloration par immunofluorescence des pièges extracellulaires des neutrophiles dans des échantillons de tissus

371 Views

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04:01 min

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August 18, 2023

DOI:

10.3791/200337-v

DOI:

10.3791/200337-v

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04:01 min

August 18, 2023

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2 Views

Lavinia Schoenfeld, Birgit Appl, Laia Pagerols-Raluy, Annika Heuer, Konrad Reinshagen, Michael Boettcher²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

Tags

Immunofluorescence Neutrophil Extracellular Traps Blocking Solution Donkey Serum Primary Antibodies Antibody Dilution Buffer Myeloperoxidase Citrullinated Histone H3 Neutrophil Elastase Secondary Antibodies DAPI Mounting Medium

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