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Immunologie und Infektion

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Staining and Imaging to Visualize Neutrophil Extracellular Traps

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Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

JoVE Journal
Immunologie und Infektion

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JoVE Journal Immunologie und Infektion

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Staining and Imaging to Visualize Neutrophil Extracellular Traps

DOI:

10.3791/200337-v

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04:01 min

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August 18, 2023

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Lavinia Schoenfeld, Birgit Appl, Laia Pagerols-Raluy, Annika Heuer, Konrad Reinshagen, Michael Boettcher²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

Tags

Immunofluorescence Neutrophil Extracellular Traps Blocking Solution Donkey Serum Primary Antibodies Antibody Dilution Buffer Myeloperoxidase Citrullinated Histone H3 Neutrophil Elastase Secondary Antibodies DAPI Mounting Medium

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