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3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

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Crypt Isolation from Murine Small Intestine (Video) | JoVE

JoVE Journal
Biologia

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JoVE Journal Biologia

Crypt Isolation from Murine Small Intestine

Crypt Isolation from Murine Small Intestine (Video) | JoVE

3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

DOI:

10.3791/200007-v

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05:06 min

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June 02, 2023

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Yuta Takase, Kazuto Fujishima³, Toshio Takahashi

¹Suntory Foundation for Life Sciences,Bioorganic Research Institute, ²Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS),Kyoto University, ³Faculty of Medicine,Osaka Medical and Pharmaceutical University

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Keywords: Crypt Isolation Murine Small Intestine PBS Antibiotics EDTA Extracellular Matrix ECM DMEM Sorbitol Cell Strainer Cell Culture Centrifugation Microscopy

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