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An In vitro FluoroBlok Tumor Invasion Assay
JoVE Journal
Biologia
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JoVE Journal Biologia
An In vitro FluoroBlok Tumor Invasion Assay

An In vitro FluoroBlok Tumor Invasion Assay

DOI:
10.3791/1475-v

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07:53 min

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July 20, 2009

•

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1BD Biosciences,Discovery Labware

Capitoli

  • 00:05Titolo
  • 00:59Introduction
  • 01:22Preparing Cells and the BD Tumor Invasion System
  • 03:30Cell Invasion, Labeling Cells, and Measuring Fluorescence
  • 05:59Cell Invasion Assay Results
  • 07:27Conclusion

Summary

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This video demonstrates how to measure cell invasion through cell culture inserts using a fluorescence-based methodology.

Tags

In Vitro FluoroBlok Tumor Invasion AssayMetastatic CellsBasement MembraneMigrateInvasive PropertyBD BioCoat Tumor Invasion SystemBD Falcon FluoroBlok 24-Multiwell Insert Plate8.0 Micron Pore Size PET MembraneBD Matrigel MatrixReconstituted Basement MembraneProtein StructureInvasion StudyCell Invasion QuantitationFluorescent Dye LabelingDiIC12(3)Calcein AMFluorescence MeasurementBD FluoroBlok Membrane

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