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Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
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Biologia
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JoVE Journal Biologia
Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium

18,862 Views

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14:16 min

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February 11, 2009

DOI:

10.3791/1009-v

DOI:
10.3791/1009-v

•

14:16 min
February 11, 2009

•

2 Views
, ,
1Department of Dermatology,Brigham and Women’s Hospital, 2Department of Dermatology,Brigham and Women’s Hospital and Harvard Medical School

Summary

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This report provides a visual depiction of parallel-plate flow chamber analysis for studying leukocyte endothelial interactions under physiologic shear stress. This method is particularly useful for investigating the role of endothelial (E)-selectin and leukocyte E-selectin ligands that trigger leukocyte rolling on endothelial cell surfaces.

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E-selectinLeukocyte RollingMicrovascular EndotheliumRecruitmentCytokinesLectin ReceptorCarbohydrate Counter-receptor LigandsAdhesive EventsDiapedesis ActivityLeukocyte TraffickingInflammationAnti-inflammatory TherapeuticsTechnologyParallel-plate Flow Chamber ApparatusDigital VisualizationRecording Software

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Wiese, G., Barthel, S. R., Dimitroff, C. J. Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium. J. Vis. Exp. (24), e1009, doi:10.3791/1009 (2009).

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