Source: Arun Kumar, S. et al., Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform. J. Vis. Exp. (2020)
This video demonstrates a method for the generation of a polymer bubble or polybubble-based novel platform that enables delayed burst release. Polyester-based polymers are used to form the polybubbles with core-shell structure, and small molecules and antigens can be used as cargo.
1. Polycaprolactone triacrylate (PCLTA) synthesis
2. Formation of the polybubble
NOTE: Injecting polymer in the deionized (DI) water would cause the polybubbles to migrate to the bottom of the vial resulting in flattened bottom. Use 10% (wt/vol) carboxymethyl cellulose (CMC) to fill the glass vial instead to avoid polybubble flattening.
3. Modulation of polybubble diameter
4. Centering cargo within polybubble
5. Cargo Formulation
NOTE: Polybubble formulation can house various cargo types, including small molecules, proteins, and nucleic acids.
6. Release of cargo
NOTE: Small molecule or antigen can be used as the cargo type
The authors have nothing to disclose.
1-Step Ultra Tetramethylbezidine (TMB)-Enzyme-Linked Immunosorbent Assay (ELISA) Substrate Solution | Thermo scientific | 34028 | |
2-Hydroxy-2-methylpropiophenone | TCI AMERICA | H0991 | |
450 nm Stop Solution for TMB Substrate | Abcam | ab17152 | |
Acryloyl chloride | Sigma Aldrich | A24109-100G | |
Acriflavine | Chem-Impex International | 22916 | |
Anhydrous ethyl ether | Fisher Chemical | E138-500 | |
Anti-HIV1 gp120 antibody conjugated to horseradish peroxidase (HRP) | |||
Bovine serum albumin (BSA) | Fisher BioReagents | BP9700100 | |
BSA-CF488 dye conjugates | Invitrogen | A13100 | |
Carboxymethylcellulose (CMC) | Millipore Sigma | 80502-040 | |
Chloroform | Fisher Chemical | C2984 | |
Coating buffer | Abcam | ab210899 | |
Dichloromethane (DCM) | Sigma Aldrich | 270997-1L | |
Diethyl ether | Fisher Chemical | E1384 | |
Legato 100 Syringe Pump | KD Scientific | 14 831 212 | |
Nonfat dry milk | Andwin Scientific | NC9022655 | |
Potassium carbonate | Acros Organics | AC424081000 | |
Phosphate saline buffer | Fisher BioReagents | BP3991 | |
(Poly(caprolactone) | Sigma Aldrich | 440744-250G | |
(Poly(caprolactone) triol | Acros Organics | AC190730250 | |
Poly (lactic-co-glycolic acid) diacrylate | CMTec | 280050 | |
Recombinant HIV1 gp120 + gp41 protein | Abcam | ab49054 | |
Trehalose | Acros Organics | NC9022655 |
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