Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates

Published: February 29, 2024

Abstract

Source: Bäuerl, C., et al. A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice. J. Vis. Exp. (2017).

This video demonstrates the detection of bacteriocin-producing lactic acid bacteria derived from mouse fecal matter. The assay determines the antimicrobial activity of bacteriocin on specific bacterial strains.

Protocol

1. LAB Counting and Bacteriocin Activity Weigh one fecal pellet of each sample in a 1.5 mL tube and add an adequate volume of ice-cold 0.9% NaCl to achieve a 10% (w/v) solution. Use sterile micro pestles for 1.5 mL tubes to homogenize the fecal suspension and prepare ten-fold serial dilutions in ice-cold 0.9% NaCl. Count the total number of LAB cells. Transfer diluted cells (100 µL) to 4 mL of prewarmed Man Rogosa Sharpe (MRS) soft agar (50 °C, 0.8% agar). Mix …

Representative Results

Figure 1: Detection of Bacteriocin Production in LAB Recovered from Feces by a Three-layer Protocol (as described in the procedure)

Divulgazioni

The authors have nothing to disclose.

Materials

Plastic Petri dish Thermo Scientific 101VR20
Brain-Heart-Infusion broth Conda 1400
European Bacteriological Agar Pronadisa 1800
Agarose D1 Low EEO Pronadisa 8010
1x TAE buffer Thermo Scientific 15558042
MRS broth Difco 288130
PBS tablets Sigma P4417-100TAB
Scale Mettler Toledo PB602-S

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Citazione di questo articolo
Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates. J. Vis. Exp. (Pending Publication), e21973, doi: (2024).

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