Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection

Published: July 31, 2023

Abstract

Source: Plaisance-Bonstaff, K., et al. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (2019)

In this video, we demonstrate the isolation of monocytes from whole human blood by immunomagnetic negative separation. The isolated monocytes can be used for further downstream analysis.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Isolation of primary human monocytes by immunomagnetic negative selection Collect 40 mL of fresh, human whole blood (from either an HIV+ patient or healthy control) in four 10 mL ethylenediaminetetraacetic acid (EDTA) vacuum tubes (…

Divulgazioni

The authors have nothing to disclose.

Materials

0.5M EDTA Invitrogen AM9260G
BD Vacutainer Plastic Blood Collection Tubes with K2EDTA BD Biosciences 366643
Easy 50 EasySep Magnet StemCell Technologies 18002
Easy Sep Direct Human Monocyte Isolation Kit StemCell Technologies 19669
PBS Gibco 20012027
RPMI 1640 with L-Glutamine Corning 10040CVMP

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Citazione di questo articolo
Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection. J. Vis. Exp. (Pending Publication), e21512, doi: (2023).

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