Ex Situ Heart Perfusion: A Technique to Preserve Isolated Porcine Heart Function

Published: April 30, 2023

Abstract

Source: Hatami, S. et. al., Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (2019).

This video describes an ex situ technique for preserving an isolated porcine heart by perfusing it with an oxygenated nutrient-rich perfusate solution. The technique allows the comprehensive evaluation of cardiac performance under physiological conditions.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Pre-surgical Preparations Place the organ chamber properly on the apparatus cart and install the silicon support membrane inside the organ chamber. The aorta (Ao), pulmonary artery (PA) and left atrium (LA) connection points can be seen in Figure 1. Install the ex situ heart perfusion (ESHP) tubing network (re…

Representative Results

Figure 1: The silicone support membrane for the heart. Support membrane pictured with integrated aortic cannula (A), left atrial cannula (B), and pulmonary artery cannula (C). Figure 2: The ESHP circuit. (A) Schematic figure of the ES…

Divulgazioni

The authors have nothing to disclose.

Materials

Debakey-Metzenbaum dissecting scissors Pilling  342202
MAYO dissecting scissors  Pilling  460420
THUMB forceps  Pilling 465165
Debakey straight vascular tissue forceps Pilling  351808
CUSHING Gutschdressing forceps  Pilling  466200
JOHNSON needle holder  Pilling  510312
DERF needle holder  Pilling  443120
Sternal saw  Stryker  6207
Sternal retractor  Pilling  341162
Vorse tubing clamp  Pilling  351377
MORRIS ascending aorta clamp  Pilling  353617
Surgical snare (tourniquet) set  Medtronic  CVR79013
2-0 SILK black 12" x 18" strands  ETHICON  A185H
3-0 PROLENE blue 18" PS-2 cutting ETHICON  8687H
Biomedicus pump drive (modified)  Medtronic 540 Modified to allow remote electronic control of pump speed
Biomedicus pump  Maquet  BPX-80
Membrane oxigenator D 905  SORIN GROUP 50513
Tubing flow module  Transonic  Ts410
PXL clamp-on flow sensor  Transonic  ME9PXL-BL37SF
TruWave pressure transducer  Edwards  VSYPX272
Intercept tubing 3/8" x 3/32" xX 6'  Medtronic  3506
Intercept tubing 1/4" x 1/16" x 8'  Medtronic  3108
Heated/Refrigerated Bath Circulator Grant  TX-150
ABL 800 FLEX Blood Gas Analyzer Radiometer  989-963
DLP cardioplegia cannula  Medtronics  20613994495406 Aortic root cannula
5F Ventriculr straight pigtail cathter  CORDIS  534550S
5F AVANTI+ Sheath Introducer  CORDIS  504605A
Emerald Amplatz Guidewire  CORDIS  502571A
Dual chamber pace maker  Medtronic 5388
Defibrilltor  CodeMaster  M1722B
Infusion pump  Baxter  AS50
Surgical electrocautery device  Kls Martin  ME411
Gas mixer  SECHRIST  3500 CP-G
Medical oxygen tank  Praxair  2014408
Carbon dioxide tank  Praxair  5823115
Bovine serum albumin  MP biomedicals  218057791

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Citazione di questo articolo
Ex Situ Heart Perfusion: A Technique to Preserve Isolated Porcine Heart Function. J. Vis. Exp. (Pending Publication), e20839, doi: (2023).

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