Corneoscleral Button Isolation: A Surgical Procedure to Obtain Corneoscleral Button from Porcine Eye
Published: April 30, 2023
Abstract
Source: Okurowska, K., et al. Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis. J. Vis. Exp. (2020).
In this video, we show a procedure to surgically isolate the corneoscleral button to generate an ex vivo porcine cornea model. The model is helpful for histological, pathological, and pharmacological studies due to its similarity to the human eye.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Sterilization CRITICAL STEP: Disinfect all forceps and scissors by soaking for 1 h in 5% (v/v) solution of Distel in distilled water, clean with a brush, rinse with tap water and sterilize in an oven at 185 °C for a minimum of 2 h. Sterilize all other glassware and reagents by autoclaving at 121 °C f…
Divulgazioni
The authors have nothing to disclose.
Materials
| Cellstar 12 well plate | Greiner Bio-One | 665180 | |
| Distel | Fisher Scientific | 12899357 | |
| Forceps | Fisher Scientific | 15307805 | |
| PBS | SLS | P4417 | |
| Petri dish | Fisher Scientific | 12664785 | |
| Povidone iodine | Weldricks pharmacy | 2122828 | |
| Scalpel blade number 15 | Fisher Scientific | O305 | |
| Scalpel Swann Morton | Fisher Scientific | 11849002 |
Tags
Citazione di questo articolo
Corneoscleral Button Isolation: A Surgical Procedure to Obtain Corneoscleral Button from Porcine Eye. J. Vis. Exp. (Pending Publication), e20831, doi: (2023).