Minimum Erythematous Dose Assay: A Method for Measuring UV Sensitivity in Mouse Models
Published: April 30, 2023
Abstract
Source: Amaro-Ortiz, A. et al. Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model. J. Vis. Exp. (2013)
This video describes a method to measure UV sensitivity in a humanized mouse model—a mouse model that mimics human skin. We determine the minimal erythematous dose (MED) of UV radiation that can cause erythema or edema on the skin. The method can help to find out the efficacy of drugs used to reduce UV sensitivity.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. Determination of UV Sensitivity by Calculation of "Minimal Erythematous Dose" (MED) Use animals that have been pre-treated with either vehicle or forskolin. Anesthetize animals with intraperitoneal injection of a standard mixture of ketamine and xylazine. Prepare a piece of UV-occlusive tape for…
Representative Results
Figure 1. Forskolin-induced melanization protects against UV-mediated inflammation as determined by minimal erythematous dose (MED) testing. (A,B) Position of UV occlusive tape and UVB doses of animals treated twice daily for 5 days (A,C) or once daily for 15 days (B,D,E) with either vehicle or forskolin. The last topical treatment was applied 48 hr prior to irra…
Divulgazioni
The authors have nothing to disclose.
Materials
| Xylazine | Anased Injection | LA04612 | Shenandoah, Iowa, USA |
| Ketamine HCl, USP | Putney | NDC 26637-411-01 | St. Joseph, MO, USA |
| Ethanol | Decon Labs. | 2705 | |
| Depilatory cream, Nair | Church Dwight | JF-11 4381322 | Priceton, NJ |
| Germicidal Hg Lamp UV-B | Westinghouse | F15T8UV-B | |
| Radiometer photometer | International light | 1LT400A | Peabody, MA,USA |
| Isothesia, Isoflurane , USP | Butler Schein | NCD 11695-6776-1 | Dublin, OH, USA |
| Chromameter | Konica Minolta | CR-400 | Ramsey, NJ, USA |
| Data Processor for Chromameter CR-400 | Konica Minolta | DR-400 | Ramsey, NJ, USA |
Citazione di questo articolo
Minimum Erythematous Dose Assay: A Method for Measuring UV Sensitivity in Mouse Models. J. Vis. Exp. (Pending Publication), e20367, doi: (2023).