Ultrasound-Guided Implantation: A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse
Published: April 30, 2023
Abstract
Source: Hay, C. A. et al. Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma. J. Vis. Exp. (2019).
This video describes the protocol for ultrasound-guided orthotopic implantation of murine-derived pancreatic ductal adenocarcinoma cells directly into the pancreas of the mouse. This causes less inflammation at the implantation site compared to surgical procedures.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the jove veterinary review board. 1. Pre-Surgical Preparation of Mice NOTE: This step is recommended to be performed 24 hours prior to the procedure. Place cages containing experimental mice on a warmer set to 37°C. Obtain new, clean cages and place on a second warming platform set to 37°C.</…
Representative Results
Figure 1: Ultrasound-guided implantation of PDA cells into the murine pancreas. (A) Orientation of mouse, ultrasound stage, and ultrasound probe used to obtain a high-resolution image of abdominal organs. Note the stage and platform have been turned 90° from the standard orientation to allow easy access to the upper left quadrant of mouse abdomen. (B) Ultrasonographic image …
Divulgazioni
The authors have nothing to disclose.
Materials
| 50 mL Conicals | Thomas Scientific | 2602A26 | |
| Blunt edged forceps | Fine Science Tools | 11000-12 | |
| Cell Dissociation Buffer | Thermo-Fisher | 13151014 | |
| Cotton Tipped swabs | Thermo-Fisher | 19062614 | |
| Covidien Monoject 3/10mL, 29G X 1/2" | Thermo-Fisher | 8881600145 | |
| Depilatory Agent | Amazon | Nair Body Lotion | |
| Forceps (blunt edge) | Fine Science Tools | 11000-12 | |
| Gauze | Fisher | 13-761-52 | |
| Gentamicin | Thermo-Fisher | 15750060 | |
| Induction Chamber | VetEquip | 941444 | |
| Isofluorane | Penn Vet Supply | VED1350 | |
| Isofluorane Vaporizer | VetEquip | 911103 | |
| L-glutamine | Thermo-Fisher | 25030081 | |
| Optixcare | MidWest Veterinary Supply | 052.50310.3 | |
| Paper Tape | Medline | MMM1530Z5 | |
| PBS | Thermo-Fisher | 14-190-250 | |
| Slide warmer | C&A Scientific | XH-2001 | |
| Sterilant (Clidox-S) | Fisher Scientific | NC0332382 (activator) NC9189926 (base) | Needs to be combined according to manufacturer's instructions |
| Sterile Alcohol prep pad | Covidien | 6818 | |
| Trypsin | Thermo-Fisher | 15090046 | |
| Ultrasound gel | Thermo-Fisher | 03-34-1LT | |
| Visualsonics Ultrasound Vevo 2100 | Visual Sonics | Vevo 2100 |
Citazione di questo articolo
Ultrasound-Guided Implantation: A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse. J. Vis. Exp. (Pending Publication), e20307, doi: (2023).