Tail Vein Injection: A Method to Administer Cancer Cells for Metastatic Studies in a Mouse Model
Published: April 30, 2023
Abstract
Source: Warren et. al., Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs. J. Vis. Exp. (2019).
This video describes a technique of injecting labeled cancer cells using tail vein injection into a mouse model. We further monitor breast cancer metastasis formation, and growth using real-time in vivo live animal imaging.
Protocol
1. Tail vein injection of labeled cancer cells NOTE: Step 1.2.4 has been optimized for 4T1 cells growing in syngeneic BALB/c mice. If other cancer cell lines and mouse strains are used, the number of cells injected, and the length of the assay should first be optimized. Expand the 4T1 cell lines generated in step 2 on two 15 cm dishes in complete growth media so that excess cells are available on the day of injection. Prepare the cells for tail vein injec…
Divulgazioni
The authors have nothing to disclose.
Materials
| 2.5% Trypsin | Gibco | 15090-046 | Trypsin for tissue culture |
| Alcohol wipes | For sterolizing the injection site before tail vein injecitons | ||
| BALB/C mice (female, 6 weeks) | Taconic | BALB-F | For tail vein metastatic colonization and burden assays |
| Dulbecco&39;s phosphate buffered saline | Himedia | TS1006 | |
| EDTA | VWR | 97061-406 | Used to dilute trypsin for tissue culture |
| FBS 100% US origin | VWR | 97068-085 | Component of complete growth media |
| L-Glutamine | Gibco | 25030-081 | Component of complete growth media |
| Mouse breast cancer cells, 4T1 | Karmanos Cancer Institute | Aslakson, CJ et al.,1992 | Mouse metastatic breast cance cell line |
| Penicillin Streptomycin | Gibco | 15140-122 | Component of complete growth media |
Citazione di questo articolo
Tail Vein Injection: A Method to Administer Cancer Cells for Metastatic Studies in a Mouse Model. J. Vis. Exp. (Pending Publication), e20228, doi: (2023).