zPDX-Analysis of Invasiveness: Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts
Published: April 30, 2023
Abstract
Source: Ren, J. et al. Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish. J. Vis. Exp. (2017).
This video describes a microinjection method for breast cancer cell delivery into the perivitelline space of transgenic zebrafish embryos. After incubation, we visualize the embryo under a fluorescent microscope to observe cancer cell invasion, dissemination, and metastasis.
Protocol
1. Image and Analyze the Metastatic Process Collect several anesthetized embryos with a wide-tip Pasteur pipette and transfer them to the glass bottom of a polystyrene dish. Remove excess water and keep a limited amount of egg water. Manipulate the embryo into position with a hair loop tool and place a cover on top of the glass. Use an inverted confocal microscope in combination with water-immersion or long-distance dry objectives. Position the embryo such that the region …
Divulgazioni
The authors have nothing to disclose.
Materials
| Wide-tip Pasteur pipette (0.5-20 µL) | Eppendorf | F276456I | |
| Confocal microscope | Leica | SP5 STED | |
| Stereo microscope | Leica | MZ16FA | |
| Tg (fli:EGFP) zebrafish strain | Kindly provided by Dr. Ewa SnaarJagalska (Institute of Biology, Leiden University, Leiden, The Netherlands) | ||
| Polystyrene dish with glass bottom | WillCo | GWST-5040 | |
| Fluorescent stereo microscope | Leica | M165 FC | |
| Tricaine (3-aminobenzoic acid) | SigmaAldrich | A-5040 |
Citazione di questo articolo
zPDX-Analysis of Invasiveness: Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts. J. Vis. Exp. (Pending Publication), e20219, doi: (2023).