JOVE JOURNAL

Immunologia e infezioni

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Journal (Immunology and Infection)

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Elizabeth Mateer¹, Slobodan Paessler¹, Cheng Huang¹

¹Department of Pathology and Institute for Human Infections and Immunity,University of Texas Medical Branch

Journal (Immunology and Infection)

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Elijah W. Chen*¹, Chyan Ying Ke*^2,3, Joanna Brzostek*¹, Nicholas R. J. Gascoigne¹, Vasily Rybakin^1,4

¹Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, ²Singapore Immunology Network,A*STAR, ³Curiox Biosystems, ⁴Department of Immunobiology, Rega Institute for Medical Research,Katholieke Universiteit (KU) Leuven

Journal (Immunology and Infection)

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Georges Khoury¹, Damian F.J. Purcell¹

¹Department of Microbiology and Immunology,University of Melbourne

Journal (Immunology and Infection)

Assaying for Inorganic Polyphosphate in Bacteria

Arya Pokhrel¹, Jordan C. Lingo², Frank Wolschendorf², Michael J. Gray¹

¹Department of Microbiology,University of Alabama at Birmingham, ²Division of Infectious Diseases,University of Alabama at Birmingham

Journal (Immunology and Infection)

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Michel Jourdan¹, Hugues de Boussac¹, Elena Viziteu¹, Alboukadel Kassambara¹, Jérôme Moreaux^1,2,3

¹IGH, CNRS,Univ Montpellier, ²Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology,CHU Montpellier, ³UFR de Médecine,Univ Montpellier

Journal (Immunology and Infection)

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Katherine Schultheis¹, Hubert Schaefer², Holly M. Pugh¹, Bryan S. Yung¹, Janet Oh¹, Jacklyn Nguyen¹, Laurent Humeau¹, Kate E. Broderick¹, Trevor R.F. Smith¹

¹Inovio Pharmaceuticals, Inc, ²Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Journal (Immunology and Infection)

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Sultan Asad^1,2, Fabiana Feitosa-Suntheimer^1,2, Alexander Gold^1,2, Berlin Londono-Renteria², Tonya M. Colpitts^1,2

¹Department of Microbiology,Boston University School of Medicine, ²National Emerging Infectious Diseases Laborator,Boston University School of Medicine, ³Department of Entomology,Kansas State University

Journal (Immunology and Infection)

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

John-William Sidhom^1,2,3, Debebe Theodros^1,2,4, Benjamin Murter^1,2, Jelani C. Zarif^1,2, Sudipto Ganguly^1,2, Drew M. Pardoll^1,2, Alexander Baras^1,2,5

¹The Bloomberg~Kimmel Institute for Cancer Immunotherapy,Johns Hopkins University School of Medicine, ²The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, ³Department of Biomedical Engineering,Johns Hopkins University School of Medicine, ⁴Department of Immunology,Johns Hopkins University School of Medicine, ⁵Department of Pathology,Johns Hopkins University School of Medicine

Journal (Immunology and Infection)

Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy

Liangfei Niu¹, Cong Wang¹, Kaile Zhang^1,2, Miaomiao Kang^1,2, Rui Liang¹, Xiaonan Zhang¹, Bo Yan¹

¹Shanghai Public Health Clinical Center,Fudan University, ²School of Life Sciences,Bengbu Medical College

Journal (Immunology and Infection)

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Chin-Wen Wei¹, Tyng-An Zhou¹, Ivan L. Dzhagalov¹, Chia-Lin Hsu¹

¹Institute of Microbiology and Immunology,National Yang-Ming University

Journal (Immunology and Infection)

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Guo Hong*^1,2, Shuangyi Fan*³, The Phyu³, Priyanka Maheshwari², Michal Marek Hoppe², Hoang Mai Phuong², Sanjay de Mel⁴, Michelle Poon⁴, Siok-Bian Ng^2,3, Anand D. Jeyasekharan^2,4

¹Department of Laboratory Medicine,AnSteel Group General Hospital, ²Cancer Science Institute of Singapore,National University of Singapore, ³Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, ⁴Department of Haematology-Oncology,National University Health System

Journal (Immunology and Infection)

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Na Tang^1,2, Yaoyao Zhang¹, Miriam Pedrera¹, Pengxiang Chang¹, Susan Baigent¹, Katy Moffat¹, Zhiqiang Shen², Venugopal Nair¹, Yongxiu Yao¹

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, ²Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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