JOVE JOURNAL

Genetica

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Journal (Genetics)

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Hogyu David Seo¹, Daeyoup Lee¹

¹Department of Biological Sciences,Korea Advanced Institute of Science and Technology

Journal (Genetics)

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Seoghyun Lee*¹, Minho Won*², Ran Hee Hwang³, Gang Min Hur², Hyunju Ro¹

¹Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, ²Department of Pharmacology, College of Medicine,Chungnam National University, ³Department of Nursing,Gwangju Women's University

Journal (Genetics)

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Olivier Loudig^1,2,3, Christina Liu^1,2, Thomas Rohan³, Iddo Z. Ben-Dov⁴

¹Department of Research,Hackensack University Medical Center, ²Department of Medical Sciences,Seton Hall University, ³Department of Epidemiology and Population Health,Albert Einstein College of Medicine, ⁴Department of Nephrology and Hypertension,Hadassah - Hebrew University Medical Center

Journal (Genetics)

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Rikke H. Kofoed¹, Cristine Betzer¹, Søren Lykke-Andersen², Ewa Molska², Poul H. Jensen¹

¹DANDRITE - Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine,Aarhus University, ²Dept. of Molecular Biology and Genetics,Aarhus University

Journal (Genetics)

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Tuesday Simmons¹, Daniel F. Caddell¹, Siwen Deng¹, Devin Coleman-Derr^1,2

¹Department of Plant and Microbial Biology,University of California, Berkeley, ²Plant Gene Expression Center,USDA ARS

Journal (Genetics)

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Jose Antonio Escudero^1,2, R Craig MacLean³, Alvaro San Millan⁴

¹Department of Animal Health,Universidad Complutense de Madrid, ²Visavet Health Surveillance Centre,Universidad Complutense de Madrid, ³Department of Zoology,University of Oxford, ⁴Department of Microbiology,Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

Journal (Genetics)

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Harriet Prior*¹, Lauren MacConnachie*¹, Jose L. Martinez¹, Georgina C.B. Nicholl¹, Asim A. Beg¹

¹Department of Pharmacology,University of Michigan

Journal (Genetics)

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Joseph C. Siefert^1,2, Emily A. Clowdus^1,2, Duane Goins¹, Amnon Koren³, Christopher L. Sansam^1,2

¹Cell Cycle and Cancer Biology Research Program,Oklahoma Medical Research Foundation, ²Department of Cell Biology,University of Oklahoma Health Sciences Center, ³Department of Molecular Biology and Genetics,Cornell University

Journal (Genetics)

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Ling Huang*¹, Xiaohui Deng*¹, Ronghua Li¹, Yanshi Xia¹, Guihua Bai², Kadambot H.M. Siddique³, Peiguo Guo¹

¹College of Life Sciences,Guangzhou University, ²Hard Winter Wheat Genetics Research Unit,United States Department of Agriculture - Agricultural Research Service, ³The UWA Institute of Agriculture,University of Western Australia

Journal (Genetics)

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Diego Marin*^1,2, Alexandra L. Nguyen*³, Richard T. Scott, Jr.^1,2, Karen Schindler³

¹IVI-RMA New Jersey, ²Jefferson College of Biomedical Sciences,Thomas Jefferson University, ³Department of Genetics,Rutgers, The State University of New Jersey

Journal (Genetics)

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Manon Torres¹, Denis Becquet¹, Séverine Guillen¹, Bénédicte Boyer¹, Mathias Moreno¹, Marie-Pierre Blanchard², Jean-Louis Franc¹, Anne-Marie François-Bellan¹

¹CNRS, CRN2M-UMR7286, Faculté de Médecine Nord,Aix-Marseille Université, ²Plate-forme d'imagerie MRI, UMS3426, CNRS 141,Institut de Génétique Humaine

Journal (Genetics)

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Lorenzo Brunetti*^1,2,3, Michael C. Gundry*^1,2,4, Ayumi Kitano⁴, Daisuke Nakada^1,2,4, Margaret A. Goodell^1,2,4,5

¹Stem Cells & Regenerative Medicine Center,Baylor College of Medicine, ²Center for Cell and Gene Therapy,Baylor College of Medicine, ³Centro di Ricerca Emato-Oncologica (CREO),University of Perugia, ⁴Department of Molecular & Human Genetics,Baylor College of Medicine, ⁵Texas Children's Hospital & Houston Methodist Hospital

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