JOVE JOURNAL

Genetica

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Journal (Genetics)

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Amy Smith¹, Mary Bergwell*¹, Ellie Smith*¹, Danita Mathew*¹, Jyoti Iyer¹

¹Department of Chemistry and Biochemistry,The University of Tulsa

Journal (Genetics)

Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Isabelle Louradour¹, Kashinath Ghosh¹, Ehud Inbar¹, David L. Sacks¹, Channa Aluvihare², Robert A. Harrell II²

¹Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health, ²University of Maryland Insect Transformation Facility,The Institute for Bioscience and Biotechnology Research

Journal (Genetics)

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Sampath Kumar Loganathan¹, Ahmad Malik^1,2, Ellen Langille^1,2, Chen Luxenburg³, Daniel Schramek^1,2

¹Centre for Molecular and Systems Biology,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ²Department of Molecular Genetics,University of Toronto, ³Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University

Journal (Genetics)

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

Payam Haftbaradaran Esfahani¹, Ralph Knöll^1,2

¹Department of Medicine, Integrated Cardio Metabolic Centre (ICMC), Heart and Vascular Theme,Karolinska Institutet, ²Bioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D,AstraZeneca

Journal (Genetics)

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Christie Dix¹, Susan Sgro¹, Anup Patel¹, Carly Perrotta¹, Noor Eldabagh¹, Katherine L. Lomauro¹, Fredy W. Miguez¹, Prianka Chohan¹, Charmi Jariwala¹, James T. Arnone¹

¹Department of Biology,William Paterson University

Journal (Genetics)

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Katsunori Yanai¹, Shohei Kaneko¹, Hiroki Ishii¹, Akinori Aomatsu^1,2, Kiyonori Ito¹, Keiji Hirai¹, Susumu Ookawara¹, Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, ²Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, ³Department of Medical Physiology,Meiji Pharmaceutical University

Journal (Genetics)

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Tadej Pajič^1,2, Tanja Belčič Mikič¹, Helena Podgornik^1,3, Jurka Klun¹, Sandra Šućurović¹, Samo Zver¹, Matjaz Sever¹

¹Department of Hematology,University Medical Centre Ljubljana, ²Clinical Institute for Genomic Medicine,University Medical Centre Ljubljana, ³Faculty of Pharmacy,University of Ljubljana

Journal (Genetics)

High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)

Kaitlin N. Montanera^1,2, Ho Sung Rhee^1,2

¹Department of Cell and Systems Biology,University of Toronto, ²Department of Biology,University of Toronto

Journal (Genetics)

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Adam Thrash¹, Marilyn L Warburton²

¹Institute for Genomics, Biocomputing & Biotechnology,Mississippi State University, ²Corn Host Plant Resistance Research Unit,USDA-ARS

Journal (Genetics)

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Gabrielle J. Benitez¹, Kosaku Shinoda^1,2,3

¹Departments of Medicine,Albert Einstein College of Medicine, ²Departments of Molecular Pharmacology,Albert Einstein College of Medicine, ³Fleischer Institute of Diabetes and Metabolism

Journal (Genetics)

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Keerat Kaur^1,2,3, Nishat Sultana^1,2,3, Yoav Hadas^1,2,3, Ajit Magadum^1,2,3, Mohammad Tofael Kabir Sharkar^1,2,3, Elena Chepurko^1,2,3, Lior Zangi^1,2,3

¹Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, ²Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Journal (Genetics)

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Andi M. Wilson^1,2, Brenda D. Wingfield^1,2

¹Department of Biochemistry, Genetics & Microbiology,University of Pretoria, ²Forestry & Agricultural Biotechnology Institute (FABI),University of Pretoria

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