JOVE JOURNAL

Biologia dello sviluppo

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Journal (Developmental Biology)

Isolation of Single Nuclei from Human Brain Organoids for Single-Nucleus RNA Sequencing

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic^1,3, Anastasiya Boltengagen¹, Agnieszka Rybak-Wolf^1,3, Nikolaus Rajewsky^1,2

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie,Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

Journal (Developmental Biology)

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Alexander Blümke^1,2, Jessica Simon¹, Elizabeth Leber¹, Marta Scatena¹, Cecilia M. Giachelli¹

¹Department of Bioengineering, Department of Medicine,University of Washington, ²Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Journal (Developmental Biology)

Preparation of Enriched Live Cell Suspension for Nuclei Isolation from iPSC-Derived Blood Cells

Rong Qiu¹, Chayanne Petit¹, Christopher S. Thom^1,2

¹Division of Neonatology,Children’s Hospital of Philadelphia, ²Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

Journal (Developmental Biology)

Isolating Nuclei from iPSC-Derived Cells for Advanced Multiomics Research

Rong Qiu¹, Chayanne Petit¹, Christopher S. Thom^1,2

¹Division of Neonatology,Children’s Hospital of Philadelphia, ²Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

Journal (Developmental Biology)

Nuclear Isolation from Cryopreserved In Vitro Derived Blood Cells

Rong Qiu¹, Chayanne Petit¹, Christopher S. Thom^1,2

¹Division of Neonatology,Children’s Hospital of Philadelphia, ²Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

Journal (Developmental Biology)

Incubation of Mouse Myoblast-Sequestered Concanavalin-A Beads with Antibodies for CUT&Tag Assay

Yuefeng Li^1,2, Xiaofen Wu^1,2, Ping Hu^1,2

¹Guangzhou Laboratory, ²The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes,The Fifth Affiliated Hospital of Guangzhou Medical University

Journal (Developmental Biology)

Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research

Yuefeng Li^1,2, Xiaofen Wu^1,2, Ping Hu^1,2

¹Guangzhou Laboratory, ²The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes,The Fifth Affiliated Hospital of Guangzhou Medical University

Journal (Developmental Biology)

Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling

Yuefeng Li^1,2, Xiaofen Wu^1,2, Ping Hu^1,2

¹Guangzhou Laboratory, ²The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes,The Fifth Affiliated Hospital of Guangzhou Medical University

Journal (Developmental Biology)

Sequestration of Mouse Myoblasts onto Activated Concanavalin-A Beads

Yuefeng Li^1,2, Xiaofen Wu^1,2, Ping Hu^1,2

¹Guangzhou Laboratory, ²The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes,The Fifth Affiliated Hospital of Guangzhou Medical University

Journal (Developmental Biology)

Early Unguided Human Brain Organoid Neurovascular Niche Modeling into the Permissive Chick Embryo Chorioallantoic Membrane

Luciano Fiore^1,2, Jan Arderiu³, Andrea Martí-Sarrias^3,4, Isabel Turpín^3,4, Ruth I. Pareja^3,5, Arcadi Navarro^5,6,7,8, Mariana Holubiec^2,9, Julieta Bianchelli⁹, Tomas Falzone^2,9, Gonzalo Spelzini^1,2, Gabriel Scicolone^1,2, Sandra Acosta^3,4

¹Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN),CONICET - Universidad de Buenos Aires, ²Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética,Universidad de Buenos Aires, ³Institute of Neurosciences, Pathology and Experimental Therapeutics Dept,University of Barcelona, ⁴Functional Neurogenomics Group, Neurodevelopmental Disorders,IDIBELL, L’Hospitalet de Llobregat, ⁵IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences,Universitat Pompeu Fabra, ⁶Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra, ⁷Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, ⁸BarcelonaBeta Brain Research Center,Pasqual Maragall Foundation, ⁹Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck

Journal (Developmental Biology)

Human Brain Organoid Grafting in Chick Embryonic Chorioallantoic Membrane for Neurovascular Modeling

Luciano Fiore^1,2, Jan Arderiu³, Andrea Martí-Sarrias^3,4, Isabel Turpín^3,4, Ruth I. Pareja^3,5, Arcadi Navarro^5,6,7,8, Mariana Holubiec^2,9, Julieta Bianchelli⁹, Tomas Falzone^2,9, Gonzalo Spelzini^1,2, Gabriel Scicolone^1,2, Sandra Acosta^3,4

¹Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN),CONICET - Universidad de Buenos Aires, ²Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética,Universidad de Buenos Aires, ³Institute of Neurosciences, Pathology and Experimental Therapeutics Dept,University of Barcelona, ⁴Functional Neurogenomics Group, Neurodevelopmental Disorders,IDIBELL, L’Hospitalet de Llobregat, ⁵IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences,Universitat Pompeu Fabra, ⁶Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra, ⁷Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, ⁸BarcelonaBeta Brain Research Center,Pasqual Maragall Foundation, ⁹Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck

Journal (Developmental Biology)

An Automated Culture System for Maintaining and Differentiating Human-Induced Pluripotent Stem Cells

Kazunori Bando¹, Hiromi Yamashita¹, Fumiyuki Hattori¹

¹Innovative Regenerative Medicine,Kansai Medical University Graduate School of Medicine

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