JOVE JOURNAL

Biochimica

Showing 193 - 204 in 776 videos.

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

Dehua Li*¹, Junze Liang*¹, Yongjian Zhang², Gong Zhang^1,2

¹Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology,Jinan University, ²Chi-Biotech Co. Ltd.

Journal (Biochemistry)

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Tran Thi Thanh Thoa¹, Albert M. Liao¹, G. Thomas Caltagirone¹

¹Aptagen LLC

Journal (Biochemistry)

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Chiranjib Banerjee¹, Brandon Wey-Hung Liauw¹, Reza Vafabakhsh¹

¹Department of Molecular Biosciences,Northwestern University

Journal (Biochemistry)

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Yesenia Rodriguez¹, Kevin John Butay*¹, Kedar Sharma*¹, Elizabeth Viverette*¹, Samuel H. Wilson¹

¹Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

Journal (Biochemistry)

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Madeline M. Mumbleau*¹, Madeline R. Meyer*¹, Ming C. Hammond¹

¹Department of Chemistry and Henry Eyring Center for Cell & Genome Science,University of Utah

Journal (Biochemistry)

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Rebecca Switzer¹, Katie A. Ward², Jessica Medrano²

¹Department of Chemistry,Bucknell University, ²Program in Cell Biology/Biochemistry,Bucknell University

Journal (Biochemistry)

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Brandy N. Curtis*¹, Ellysa J. D. Vogt*², Kevin S. Cannon^1,3, Amy S. Gladfelter^1,2,3

¹Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, ²Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, ³Department of Biology,University of North Carolina at Chapel Hill

Journal (Biochemistry)

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

Pushpanjali Soppina^1,2, Dipeshwari J. Shewale¹, Pradeep K. Naik², Virupakshi Soppina¹

¹Discipline of Biological Engineering,Indian Institute of Technology Gandhinagar, ²Department of Biotechnology and Bioinformatics,Sambalpur University

Journal (Biochemistry)

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

Chelcie F. Heaney^1,2, Colin J. McArdle², Kimberly F. Raab-Graham^1,2

¹Wake Forest Translational Alcohol Research Center,Wake Forest School of Medicine, ²Physiology and Pharmacology,Wake Forest School of Medicine

Journal (Biochemistry)

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

Jessica L. Henty-Ridilla¹

¹Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology,State University of New York (SUNY) Upstate Medical University

Journal (Biochemistry)

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Liza-Anastasia DiCecco*^1,2, Samantha Berry*¹, G. M. Jonaid*^1,3, Maria J. Solares^1,4, Liam Kaylor^1,4, Jennifer L. Gray⁵, Carol Bator⁶, William J. Dearnaley¹, Michael Spilman⁷, Madeline J. Dressel-Dukes⁸, Kathryn Grandfield², Sarah M. McDonald Esstman⁹, Deborah F. Kelly^1,5,6

¹Department of Biomedical Engineering,Pennsylvania State University, ²Department of Materials Science and Engineering,McMaster University, ³Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, ⁴Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, ⁵Materials Research Institute,Pennsylvania State University, ⁶Huck Institutes of the Life Sciences,Pennsylvania State University, ⁷Applications team,Direct Electron, ⁸Application Scientist,Protochips, Inc., ⁹Department of Biology,Wake Forest University

Journal (Biochemistry)

Inner Mitochondrial Membrane Sensitivity to Na⁺ Reveals Partially Segmented Functional CoQ Pools

Pablo Hernansanz-Agustín¹, José Antonio Enríquez^1,2

¹Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, ²Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

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