Encyclopedia Of Experiments

Assay Techniques

This collection features various bioanalytical techniques to assess the activity, potency, or localization of test substances in living organisms performed at the macromolecular, sub-cellular, cellular, or organism level. Assay outcomes are detected directly or indirectly by visualizing the responses via colorimetric, enzymatic, or electrophysiological measurement methods.

Encyclopedia Of Experiments

Assay Techniques

This collection features various bioanalytical techniques to assess the activity, potency, or localization of test substances in living organisms performed at the macromolecular, sub-cellular, cellular, or organism level. Assay outcomes are detected directly or indirectly by visualizing the responses via colorimetric, enzymatic, or electrophysiological measurement methods.

Sections

Biochemical assays

Cell-based assays

Immunoassays

Biochemical assays

Cell-based assays

Immunoassays

Biochemical assays

Phospholipase Activity Assay

A Gel Diffusion Assay to Determine Phospholipase Activity in Crude Venom Extract from Anthopleura dowii

Ring-Shaped Micropatterning Cell Chirality Assay

An In Vitro Technique to Determine Multicellular Chirality Based on Cell Alignment on Ring-Shaped Micropatterns

Citrate Synthase Activity Assay

A Colorimetric Assay to Measure Mitochondrial Citrate Synthase Activity in Drosophila Tissue Homogenate

Bathophenanthroline Sulfonate-Based Colorimetric Assay

A Simple and Rapid Method for Quantitation of Non-Heme Iron in Mouse Liver Tissue

Substrate Oxidation Assay in Cultured Cells

An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO₂

Planar Supported Lipid Bilayer Assay

A Microscopy-Based In Vitro Technique to Investigate Septin Assembly on Biological Membrane Mimics

Spherical Supported Lipid Bilayer Assay

A Technique to Observe the Septin Assembly on Microspheres

Small Molecule Nematicides Screening Assay

A Medium Throughput Method to Screen Potential Nematicides Against Ditylenchus dipsaci

In Vitro Mineralization Assay

A Colorimetric Method to Quantify Osteoblast Calcium Deposits on Bone Graft Substitute by Alizarin Red S Staining and Extraction

Larval Contact Assay

A Procedure to Evaluate Mosquito Larval Mortality following Direct Contact with Test Larvicidal Compound

Topical Testing Assay

A Technique to Evaluate the Insecticidal Potential of Dopamine Receptor Antagonists on Adult Female Mosquitoes by Topical Application

Mosquito Feeding Assay

A Method to Evaluate Test Compound Effects on Mortality and Fecundity of Adult Female Mosquitoes

Protein Aggregate Formation Assay

A Method to Detect and Quantify Protein Aggregation in Cultured Cells upon Induction by Proteasome Inhibitor

Endotoxin Activity Assay

A Chemiluminescence-Based Bioassay for Investigating Endotoxin Activity in Blood Sample

Turbidimetric Limulus Amoebocyte Lysate Assay

A Technique to Detect and Quantify Endotoxin Contamination in Nano-Formulated Drugs

Gel-Clot Limulus Amoebocyte Lysate Assay

A Method for Bacterial Endotoxin Detection in Nanoparticle Formulations

Chromogenic Polymer Hydrogel-Based Enzyme Screening Assay

A High Throughput Method to Screen the Carbohydrate-Active Enzymes Using Synthetic Substrates

Insoluble Chromogenic Biomass-Based Enzyme Screening

An Assay to Evaluate the Degradation Activity of Plant Biomass-Degrading Enzymes

Differential Nuclear Staining Assay

An Assay to Determine Mitocan Cytotoxicity by Propidium Iodide and Hoechst Double Staining

The Gliding Actin Filament Assay

An In Vitro Motility Assay to Study Translocation of Actin Filaments on Immobilized Myosins

Inverted Motility Assay

An In Vitro Technique to Visualize Myosin Movement on Immobilized Actin Filaments

Differential Radial Capillary Action of Ligand Assay

A High-Throughput Technique to Identify Bacterial Nucleotide Second Messenger-Binding Intracellular Proteins

Misfolding-Prone Protein Degradation Assay

A Technique to Monitor Misfolded Protein Degradation Using Cycloheximide Treatment and Detergent Fractionation

Fluorescence Microplate-Based Cycloheximide Chase Assay

A Technique to Monitor the Degradation Kinetics of Fluorescent Nuclear Misfolded Proteins

The Luciferase Assay

A High-Throughput Assay to Measure Luminescent Signals Using Engineered Nanoluciferase System-Based Bioreporters

Soluble Protein Assay to Quantify Soluble Protein Content in Plant Tissues

Fluorescent Reporter-Based Paralysis Assay

A Technique to Assess Age-Associated Progressive Formation of Polyglutamine Fluorescent Reporter and Associated Paralysis in Caenorhabditis elegans

Colorimetric Assay for the Quantification of Non-Structural Carbohydrates in Plant Tissues

Luciferase-Based Growth Assay to Quantify Intracellular Parasite Reproduction

Amplified Luminescent Proximity Homogeneous Assay

A Bead-Based Proximity Assay to Screen Small Molecules Inhibiting Protein-Protein Interactions

Luciferase Assay to Study Efficacy of Antiparasitic Chemicals Against Parasites

Gut Acidification Monitoring Assay

A Technique to Study the Acidification of the Intestinal Lumen in Drosophila using Bromophenol Blue Dye

Msp1 Extraction Assay to Study the Removal of Mislocalized TA Proteins

Mitochondrial Calcium Uptake Assay

A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria

β-Galactosidase Assay to Evaluate Drug-Induced Cytotoxicity in Parasites

A Plate-Based Assay to Measure Monoamine Release from Brain Slices on Test Drug Exposure

PolyQ Aggregation Assay to Identify Neuroprotective Effect of a Compound in Worms

An Assay to Study Effect of Test Compound against PolyQ-Mediated Neurotoxicity in Worms

Examine Chemoavoidance Behavior in Transgenic Worms Using Osmotic Avoidance Assay

Colorimetric Assay for Quantification of Lactate in Test Samples

Pyruvate Assay for Estimation of Pyruvate Levels in Cellular Fractions of Worms

Spectrophotometric Assay to Determine Glycogen Synthase Activity

Glycogen Branching Assay to Measure the Degree of Glycogen Branching

Thiobarbituric Acid-Reactive Substances Assay to Assess Oxidative Stress in Human Serum

Split Luciferase Complementation Assay to Identify Specific Protein-Protein Interactions

Cell-based assays

Extracellular Vesicle Uptake Assay

A Method to Visualize and Quantify Cellular Uptake of Extracellular Vesicles Using 3D Fluorescence Imaging Technique

Three-Chambered Array-Based Impedance Assay

A Real-Time Analysis Technique to Assess Invasive Potential of Cancer Cells by Measuring Electrical Impedance

Lysostaphin-Based Enzyme Protection Assay

An In Vitro Method to Quantify Intracellular Staphylococcus aureus Load by Enzyme-Mediated Selective Killing of Extracellular Bacteria

Resorption Pit Assay

An In Vitro Technique to Quantify Calcium Resorption Activity of Mature Osteoclasts using Calcium Phosphate-Coated Culture Plates

Protein Phase Separation Assay

An Optogenetic Method for Mutant RNA-Binding Protein Phase Separation in Spinal Motor Neurons of Zebrafish Larvae

Bead Sprouting Assay for Early-Stage Angiogenesis Assessment

An In Vitro Bead-Based Model to Study Endothelial Sprouting in the Presence of Pericytes

Hemolysis Assay

An In Vitro Method to Measure Calcium Ionophore-Induced Lysis in Human Erythrocytes

Annexin V Binding Assay

A Fluorescence-Based Technique to Identify Apoptotic Erythrocytes via Phosphatidylserine Labeling

Caco-2 Cell Bioassay

An In Vitro Method for Measuring Iron Bioavailability in Complex Food Sources

In Vitro Glucose Depletion Assay

A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement

Measurement of Extracellular Fluorescent Glucose Analog Depletion

A Surrogate Measurement Technique to Assess Glucose Uptake in Mouse Organs Ex Vivo

Measuring SMC Contraction In Vitro

A Non-Invasive Method to Evaluate Smooth Muscle Cells Contraction using Electric Cell-Substrate Impedance Sensing

Humanized Mediator Enzyme Release Assay

An Immunochemical Method to Screen Allergic Potential of Test Antigen by Monitoring Basophil Degranulation Response In Vitro

ATP-Based Luciferase Viability Assay

A Homogenous Method to Evaluate the Growth-Inhibitory Potential of Test Agents on Tumor Organoids

Fluorescent Probe Imaging Assay

A Technique to Visualize Oxidative Stress in the Reactive Oxygen Species Inducer-Treated Cultured Intestinal Organoid Cells

Flow Cytometry Assay to Quantify Oxidative Stress

An Assay to Quantify Reactive Oxygen Species in Intestinal Organoids Using Fluorogenic Probes

In Vitro Persister Assay

A Method to Evaluate the Effect of Osmolytes on Bacterial Persistence

Calcium Influx Assay to Measure Mitochondrial Calcium Uptake in Cultured Cells

Bacterial Co-Incubation Assay

A Fluorescence Microscopy-Based Technique to Visualize Intraspecific Bacterial Competition at the Single-Cell Level

Fluorescence Leakage Assay to Study Cell-Penetrating Peptide Interaction with Membrane

Virus Plaque Assay

A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells

Calcification Assay to Measure Calcium Concentration in Aortic Valve Interstitial Cells

Terminal Transferase-Mediated dUTP Nick End-Labeling—TUNEL Assay

An In Situ Method to Detect DNA Fragmentation in Apoptotic Cells

Electroencephalography-Based Recording Technique to Record the Epileptiform Activity

Caspase-3/7 Assay

A Luminescence-Based Assay to Quantify Apoptosis by Measuring Caspase-3/7 Activities in Frog Skin Explants

Wound Assay to Evaluate the Migratory Capacity of Murine Myoblasts in Co-Culture

The Phagocytosis Assay

An Immunostaining Technique to Visualize Apoptotic Cell Engulfment in the Embryonic Cells of Drosophila melanogaster

Comet Assay for DNA Damage Detection in Cells

Fixed Cell Efferocytosis Assay

A Method to Study Efferocytic Uptake of Apoptotic Cells by Macrophages Using Fluorescence Microscopy

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

The In Vitro Single Oocyte Reporter Assay

A Technique to Study Target mRNA Translation Regulation During In Vitro Oocyte Maturation

Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time

alamarBlue Assay

A Sensitive Fluorometric Method to Screen the Cytotoxic Effects of Artificial Tear Formulations on Metabolic Activity of Human Corneal Epithelial Cells

Impedance-Based Cellular Assay to Measure Multiplicity of Infection of Influenza Virus

Cellular Impedance-Based Survival Kinetics Assay to Assess Virus Survival

Choroid Sprouting Assay to Study Ocular Microvascular Proliferation Ex Vivo

Estrogen Receptor-Reporter Activity Assay to Screen Phytoestrogen Estrogenic Activity

Time-Resolved Forster Resonance Energy Transfer for Monitoring Protein Phosphorylation in Cells

Luminescence-Based Assay to Assess Neurotoxicity of Test Compounds on Neurons

Immunoassays

Multianalyte Immunobead Assay

A Flow-Based Immunoassay Technique for Concurrent Detection of Multiple Antibody Subtypes in Human Serum Sample

The Virus-Like Particles Capture Assay

A Method to Isolate Antigen-Displaying VLPs from a Sample Using Neutralizing Antibody-Conjugated Magnetic Beads

Single-Molecule Pull-Down Assay for Protein Phosphorylation Analysis

A High Throughput Technique to Quantify Protein Phosphorylation in Cell Lysate

SUMOylation Assay

An In Vitro Technique to Detect the SUMOylation Status of Substrate Proteins by Immunoblotting

Bioreporter Assay

A Sensitive Technique using a Bioluminescent Reporter System to Detect SARS-CoV-2 Antibodies

Immunofluorescence Assay to Detect PM_2.5-Mediated DNA Damage in Fish Embryos

Cell-Based AAV Neutralizing Antibody Assay

A Colorimetric Technique to Detect Neutralizing Antibodies Against Specific Adeno-Associated Viruses in Serum

Neuronal Death Assay to Evaluate Cell Death in Ex Vivo Epilepsy Model

Enzyme-Linked Immunosorbent Assay to Verify Chemically-Coupled Antibody-Antigen Conjugates

Immunofluorescence Assay to Evaluate Effect of Target Proteins on Neurite Outgrowth

Electrochemiluminescence Assay for Quantification of Target Protein Levels in Brain Lysate