Encyclopedia Of Experiments

Gastrointestinal Cancer

Gastrointestinal cancers include various tumor types originating in the regions or organs associated with the gastrointestinal tract. The collection comprises research protocols on cell isolation, culturing, and adhesion studies, tissue staining and histological analysis, DNA methylation studies, tissue preservation methods, exosome analysis, and generation of animal models.

Encyclopedia Of Experiments

Gastrointestinal Cancer

Gastrointestinal cancers include various tumor types originating in the regions or organs associated with the gastrointestinal tract. The collection comprises research protocols on cell isolation, culturing, and adhesion studies, tissue staining and histological analysis, DNA methylation studies, tissue preservation methods, exosome analysis, and generation of animal models.

Sections

Assays and techniques

Animal model studies

Assays and techniques

Animal model studies

Assays and techniques

Exosome Block Preparation

A Protocol to Preserve Exosomes From Cultured Human Colon Cancer Cells

Time-lapse Fluorescence Microscopy of Neutrophil Extracellular Traps or NETs

A Technique to Analyze Post-adhesion Interaction between Cancer Cells and NETs

Sectioning, Staining and TEM Imaging of Embedded Exosomes

A Protocol To Visualize the Structural Features of Exosomes Using TEM

Lamina Propria Mononuclear Cell Isolation

A Silica-based Density Gradient Centrifugation Technique to Obtain Mononuclear Cells from Murine Colon

Splitting Spheroids for Sub-culturing and Shipping

A Procedure for Propagating and Transferring Spheroids from Small Bowel Neuroendocrine Tumor

Vitrification-based Cryopreservation

An Ultra-rapid Cooling Technique to Preserve Biological Specimens at Very Low Temperatures for Long-term Storage

Peritoneal Low-density Neutrophil Isolation

A Technique to Obtain Low-density Neutrophils from Peritoneal Lavage Fluid Using Magnetic Activated Cell Sorting

Negative Staining of Purified Exosomes

A Procedure to Visualize Exosome Morphology Using Transmission Electron Microscopy

Fluorescence Microscopy of Neutrophil Extracellular Traps or NETs

A Technique to Observe Adhesive Interaction Between Cancer Cells and NETs

Whole Mount Preparation of Mouse Intestine

Using a Simple Gut-incising Device to Prepare Whole Mount

Deparaffinization and Rehydration of Tissue Sections

A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration

FFPE Tumor Tissue Macrodissection

A Technique to Obtain Specific Tumorous Tissue from Unstained Specimens

Bisulfite Conversion of Genomic DNA

A Method to Study DNA Methylation in Genomic DNA Samples from Gastrointestinal Cancer Tissue

Elastic Fiber Staining of Slide Sections

A Technique to Observe Gastric Cancer Metastasis

Gold Immunostaining of Exosomal Sections

A Technique to Visualize Diagnostic Markers in Exosomal Lumen

Gastric Fundic Glands Isolation

A Method to Isolate Fundic Glands from Human Stomach Tissue

Animal model studies

Snap Freezing of Muscle Tissue for Sectioning

A Protocol to Obtain Ultrathin Sections of Rapidly Frozen Murine Muscle Tissue

Hindlimb Skeletal Muscle Extraction

A Procedure to Excise Hindlimb Skeletal Muscles from Murine Model

Murine Vertical Sleeve Gastrectomy

A Surgical Procedure to Downsize Stomach in Experimental Mouse Models

Intestinal Crypts Isolation

A Method to Isolate Whole Crypts from Small Intestine of Murine Model