Encyclopedia Of Experiments

Pancreatic Cancer

The collection features protocols demonstrating the generation of pancreatic animal models, genetic engineering of pancreatic cancer cells, methods for in vitro modeling of pancreatic cancer, cell isolation & characterization techniques, and tumor cell behavior and response studies.

Encyclopedia Of Experiments

Pancreatic Cancer

The collection features protocols demonstrating the generation of pancreatic animal models, genetic engineering of pancreatic cancer cells, methods for in vitro modeling of pancreatic cancer, cell isolation & characterization techniques, and tumor cell behavior and response studies.

Sections

Assays & techniques

Cell isolation & culture

Assays & techniques

Cell isolation & culture

Assays & techniques

DCE-MRI of Orthotopic Pancreatic Tumor Xenografts

A Method to Assess Microvasculature in a Target Tumor Tissue

Imaging Calcium Dynamics in Pancreatic Cells

A Technique to Study Real-time Changes in Cytosolic Calcium Concentration in Pancreatic Islet

Immunofluorescent Staining of Spheroids

A Technique to Analyze Spheroids for Expression of Intra- and Extracellular Antigen Expression

Bioluminescent Orthotopic Pancreatic Cancer Mouse Model

A Non-invasive Technique to Monitor Cancer Progression in Mice

Ex Vivo T Cell Stimulation in the Pancreatic Tumor

A Method to Increase Cytokine Production in T Cells

Extracellular Flux Assay in 3D Spheroids

A Method for Measuring the Metabolic Activity of Pancreatic Tumor Spheroids

Ultrasound-Guided Implantation

A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse

Grafting Pancreatic Islets

A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging

Orthotopic Homograft Tumor Transplantation

A Technique to Generate Pancreatic Tumor Mouse Models

Irreversible Electroporation

A Technique for Ablating Pancreatic Tumor in Mouse

Intracardiac Delivery of Viral Vectors

A Technique to Inject Viral Vectors Intracardially to Study Pancreatic Metastasis in Murine Model

Fluorescent Orthotopic Mouse Model

Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo

Sphere Formation Assay

An In Vitro Method to Identify Pancreatic Cancer Stem Cells and Screen Therapies

In Vitro Pancreatic Neuroplasticity Assay

Studying Changes in GI Tract Neurons Exposed to Healthy and Cancerous Human Pancreatic Tissue Extracts

PTM Based Tumor Microenvironment Monitoring

A Method for Tracking Changes in ECM Rigidity Using Tumor Spheroids

Organoid Electroporation

A Method to Transfect Plasmid DNA into Gastrointestinal Organoids

Cell isolation & culture

Magnetically Bioprinted 3D Spheroid Co-cultures

A Method for Co-culturing Cancerous Cells and Fibroblasts

Acinar-to-Ductal Metaplasia Induction

A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture

Pancreatic Islets Isolation through Collagenase Perfusion

An Enzymatic Method to Isolate Pancreatic Islet Cells

Primary Acinar Cell Isolation

A Method to Isolate Acinar Cells from Mouse Pancreas

PDAC Stem Cell Isolation

A Method to Isolate Cancer Stem Cells from Pancreatic Tumors