MultiBac System-Based Purification and Biophysical Characterization of Human Myosin-7a

Marvin Wright; Shayna Redford; Jacob Vehar; Kevin C. Courtney; Neil Billington; Rong Liu

doi:10.3791/67135

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Biochimica

Recombinant Human Myosin-7a Production and Functional Characterization Protocol

August 23, 2024

Author Spotlight: Unraveling the Role of Myosin-7a and Usher Proteins in Hearing and Human Disease

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Marvin Wright*¹, Shayna Redford*¹, Jacob Vehar¹, Kevin C. Courtney¹, Neil Billington^1,2, Rong Liu¹

¹Department of Biochemistry & Molecular Medicine, School of Medicine,West Virginia University, ²Microscope Imaging Facility,West Virginia University

Summary

This protocol details the procedures for recombinantly producing the human myosin-7a holoenzyme using the MultiBac Baculovirus system and for studying its motility using a tailored in vitro filament gliding assay.

Recombinant Human Myosin-7a Production and Functional Characterization Protocol

Author Spotlight: Unraveling the Role of Myosin-7a and Usher Proteins in Hearing and Human Disease

Summary

Article