Recording Neuronal Activity During Odor Plume Navigation with Miniscope Imaging
1Department of Cell and Developmental Biology,University of Colorado Anschutz Medical Campus, 2Neurotechnology Center,University of Colorado Anschutz Medical Campus, 3Department of Bioengineering,University of Colorado Anschutz Medical Campus, 4Civil, Environmental & Architectural Engineering,University of Colorado, 5Neuroscience Graduate Program,University of Colorado Anschutz Medical Campus
Summary
This protocol investigates the brain-behavior relationship in hippocampal CA1 in mice navigating an odor plume. We provide a step-by-step protocol, including surgery to access imaging of the hippocampus, behavioral training, miniscope GCaMP6f recording and processing of the brain, and behavioral data to decode the mouse position from ROI neural activity.
