Methodology for Studying Interactions of Vitamin A Membrane Receptors and Opsin Protein with their Ligands in Generating the Retinylidene Protein

Rakesh Radhakrishnan; Anjelynt Lor; Dorothy Li; Deepti Mudaliar; Glenn P. Lobo

doi:10.3791/67036

Rivista

Biochimica

Investigating Vitamin A Receptor Kinetics and Function for Retinoid Homeostasis

October 04, 2024

Author Spotlight: Unraveling Vitamin A Transport Mechanisms - Linking Liver Receptors to Vision Health through RBPR2 and RBP4 Interactions

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Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Deepti Mudaliar², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences,University of Minnesota, ²High Throughput Screening Laboratory, Institute for Therapeutics Discovery & Development,University of Minnesota

Summary

Here, we describe two quantitative methods for studying the protein-ligand interactions of vitamin A membrane receptors and photoreceptor opsin with their respective physiological ligands.

Investigating Vitamin A Receptor Kinetics and Function for Retinoid Homeostasis

Author Spotlight: Unraveling Vitamin A Transport Mechanisms - Linking Liver Receptors to Vision Health through RBPR2 and RBP4 Interactions

Summary

Article