Assessment of DNA Double Strand Break Repair Activity Using High-throughput and Quantitative Luminescence-Based Reporter Assays

Diego Grande; Eeson Rajendra; Bethany Mason; Alessandro Galbiati; Simon J. Boulton; Graeme C. M. Smith; Helen M. R. Robinson

doi:10.3791/66969

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Quantifying DNA Double Strand Break Repair with Nanoluciferase Reporter Assays

June 14, 2024

Author Spotlight: Developing Novel Anticancer Therapeutics Targeting the DNA Damage Response

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Transient Transfection of Extrachromosomal Reporter Substrates and Luminescence Detection to Study DNA Double Strand Break Repair Pathways

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Diego Grande*¹, Eeson Rajendra*¹, Bethany Mason¹, Alessandro Galbiati¹, Simon J. Boulton^1,2, Graeme C. M. Smith¹, Helen M. R. Robinson¹

¹Artios Pharma Ltd, Cambridge, ²The Francis Crick Institute, London

Summary

We present protocols for the assessment of double strand break repair pathway proficiency in cells using a suite of luminescence-based extrachromosomal reporter substrates.

Quantifying DNA Double Strand Break Repair with Nanoluciferase Reporter Assays

Author Spotlight: Developing Novel Anticancer Therapeutics Targeting the DNA Damage Response

Transient Transfection of Extrachromosomal Reporter Substrates and Luminescence Detection to Study DNA Double Strand Break Repair Pathways

Summary

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