Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers

Daniel S. Shin; Juliana Barreto de Albuquerque; James J. Moon

doi:10.3791/66939

Rivista

Immunologia e infezioni

Isolation and Characterization of Antigen-Specific T Cells in Mouse Lungs

July 19, 2024

Author Spotlight: Optimized Protocol for Detecting Antigen-Specific T Cells in Mouse Lungs Using Tetramers

02:10 min

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275 Views

Generating Single-Cell Suspension from Lung Tissue for Identifying Rare Antigen-Specific T-Cell Populations

03:21 min

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Enriching the Lung Sample for T Cells via Magnetic Bead Enrichment

02:19 min

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Staining the Antigen-Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting

01:37 min

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30 Views

Daniel S. Shin^1,2,3, Juliana Barreto de Albuquerque^1,3, James J. Moon^1,3,4

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, ²Division of Immunology,Boston Children’s Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Summary

We provide a detailed protocol for isolating and identifying rare antigen-specific T cell populations in mouse lungs through magnetic bead-based T cell enrichment and peptide:major histocompatibility complex (MHC) tetramers.