Mitochondrial Fractionation in Small Tissue Samples for Supercomplex Analysis

Mitochondrial Fractionation in Small Tissue Samples for Supercomplex Analysis

May 03, 2024

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Isolation of Enriched Mitochondrial Fractions from Cell Cultures and Small Animal Tissue Samples for Gel Activity Assays

Analyzing Mitochondrial Supercomplexes in Mammalian Tissue Fractions via In-Gel Activity Assays

Raquel Moreno-Loshuertos^1,2, Patricio Fernández-Silva^1,2

¹Department of Biochemistry,University of Zaragoza, ²Institute for Biocomputation and Physics of Complex Systems,University of Zaragoza

Summary

This protocol describes a technique for the analysis of respiratory supercomplexes when only small amounts of samples are available.

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