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Neuroscienze
Long-Term Engraftment of h-SC-NES Cells in Spinal Cord Organotypic Cultures

Long-Term Engraftment of h-SC-NES Cells in Spinal Cord Organotypic Cultures

April 12, 2024

Author Spotlight: Long-Term Spinal Cord Slice Culture for Advancing Spinal Cord Regeneration Therapies

01:22 min
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Isolation and Slicing of Mouse Spinal Cord Lumbar Region to Generate Organotypic Slices for Regenerative Therapy Tests

03:00 min
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137 Views

Transplantation of Human SC-NES Cells into Organotypic Slices for Validating Spinal Cord Regeneration Therapies

01:37 min
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134 Views

Immunofluorescence Staining of Organotypic Slices with Stem Cell Transplants for Confocal Microscopy

01:50 min
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127 Views
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1Department of Biology,University of Pisa

Summary

In this paper, we provide a reproducible method to generate and maintain long-term spinal cord organotypic slices transplanted with neural stem cells as an ex vivo model for testing cellular replacement therapies.

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