Enhancing Cryo-EM Map Quality with CryoSieve Particle Selection

May 10, 2024

Enhancing Density Maps by removing the majority of particles in single particle cryyogenic electron microscopy final stacks(단일 입자 극저온 전자 현미경 최종 스택에서 대부분의 입자를 제거하여 밀도 맵 향상)

Cryo-Electron Microscopy에서 향상된 입자 선별을 위한 CryoSieve 구성 및 활용

Mengjia Cai*¹, Jianying Zhu*², Qi Zhang*^3,4,5,6, Yu Xu, Zuoqiang Shi⁸, Chenglong Bao^8,9, Mingxu Hu^5,6

¹Institute of Bio-Architecture and Bio-Interactions (IBABI),Shenzhen Medical Academy of Research and Translation, ²Yau Mathematical Sciences Center, ³Key Laboratory of Protein Science (Tsinghua University),Ministry of Education, ⁴School of Life Science,Tsinghua University, ⁵Beijing Advanced Innovation Center for Structural Biology, ⁶Beijing Frontier Research Center for Biological Structure, ⁷Department of Mathematical Sciences,Tsinghua University, ⁸Yanqi Lake Beijing Institute of Mathematical Sciences and Applications, ⁹State Key Laboratory of Membrane Biology, School of Life Science

Summary

Cryo-EM을 위한 고급 입자 선택 방법인 CryoSieve는 실제 데이터 세트에 적용하여 입증된 바와 같이 최종 스택에서 대부분의 입자를 제거하여 밀도 맵 해상도를 향상시킵니다.

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