Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue

Oliver  I. Brown; Katherine I. Bridge; Sam Straw; Natallia Makava; Jason Scragg; Sunti Limumpornpetch; Cheukyau Luk; Jessica Smith; Anna Skromna; Alexander F. Bruns; Piruthivi Sukumar; Lee D. Roberts; Richard Cubbon; Klaus K. Witte; Stephen Wheatcroft; Mark T. Kearney

doi:10.3791/66608

Rivista

Medicina

Human SATMVECs: Isolation and Adipocyte Cross-Talk Studies

April 05, 2024

Author Spotlight: Insights into Cardiometabolic Diseases with Subcutaneous Adipose Tissue Microvasculature Studies

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Oliver I. Brown¹, Katherine I. Bridge¹, Sam Straw¹, Natallia Makava¹, Jason Scragg¹, Sunti Limumpornpetch¹, Cheukyau Luk¹, Jessica Smith¹, Anna Skromna¹, Alexander F. Bruns¹, Piruthivi Sukumar¹, Lee D. Roberts¹, Richard Cubbon¹, Klaus K. Witte¹, Stephen Wheatcroft¹, Mark T. Kearney¹

¹Leeds Institute of Cardiovascular and Metabolic Medicine,University of Leeds

Summary

Here, we describe a protocol for isolating, culturing, and phenotyping microvascular endothelial cells from human subcutaneous adipose tissue (hSATMVECs). Additionally, we describe an experimental model of hSATMVEC-adipocyte cross-talk.

Human SATMVECs: Isolation and Adipocyte Cross-Talk Studies

Author Spotlight: Insights into Cardiometabolic Diseases with Subcutaneous Adipose Tissue Microvasculature Studies

Summary

Article