1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
Nous présentons un protocole d’assemblage modulaire basé sur CRISPR (CRISPRmass), une méthode pour la construction à haut débit d’une bibliothèque de plasmides UAS-ADNc/ORF chez la drosophile en utilisant des ressources d’ADNc/ORF accessibles au public. CRISPRmass peut être appliqué à l’édition de diverses banques de plasmides.
