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Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein
April 26, 2024
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Summary

This article provides a procedure for the affinity purification of a human recombinant protein, flap endonuclease 1 (FEN1), which has been labeled with a 6X-histidine tag. The protocol involves the utilization of two distinct immobilized metal ion columns for the purification of the tagged protein.