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Studying Protease-Activated Receptor Ligands in Endothelial Cells

Studying Protease-Activated Receptor Ligands in Endothelial Cells

May 24, 2024

Author Spotlight: Developing Parmodulins to Target Protease-Activated Receptors for Inflammation Control

01:27 min
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Culturing the Endothelial Cell Line in 96-Well Plates for the Calcium Mobilization Assay

02:49 min
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11 Views

Enhanced Plate Reader Assay for Calcium Mobilization in Endothelial Cells

03:01 min
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11 Views
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1Function Therapeutics, 2Department of Chemistry,Marquette University, 3School of Medicine,Washington University

Summary

An improved protocol for a calcium mobilization assay with endothelial cells, used to identify ligands of protease-activated receptors (PARs), has been developed. The new protocol reduces total assay time by 90-120 min and yields reproducible concentration-response curves.

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