High-Throughput Assay to Measure Caenorhabditis elegans Food Intake and Lifespan

High-Throughput Assay to Measure Caenorhabditis elegans Food Intake and Lifespan

February 23, 2024

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Preparation of OP50 for Caenorhabditis elegans Culture

Synchronous Caenorhabditis elegans Culture Preparation and Measurement of Bacterial Clearance for Food Intake Assay

Christina Clark*^1,2,3, Alan To*^1,2,3, Michael Petrascheck^1,2,3

¹Department of Molecular and Cellular Biology,The Scripps Research Institute, ²Department of Molecular Medicine,The Scripps Research Institute, ³Department of Neuroscience,The Scripps Research Institute

Summary

This protocol describes an assay to quantify Caenorhabditis elegans feeding rate based on measuring the clearance of bacteria in liquid culture.

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