Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells <em>via</em> Transendothelial Electrical Resistance Detection

Fuhan Fan; Hong Jiang; Ya Hou; Yi Zhang; Qipeng Zhao; Yong Zeng; Xianli Meng; Xiaobo Wang

doi:10.3791/65938

Rivista

Neuroscienze

Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

September 29, 2023

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Culture of bEnd.3 Cells and Cell Viability After CoCl2 Treatment

02:32 min

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Fuhan Fan¹, Hong Jiang¹, Ya Hou¹, Yi Zhang^1,2,3, Qipeng Zhao⁴, Yong Zeng^1,2, Xianli Meng^1,2,5, Xiaobo Wang²

¹School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine, ²Meishan Hospital of Chengdu University of Traditional Chinese Medicine, ³School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine, ⁴School of Pharmacy,Ningxia Medical University, ⁵Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline,Chengdu University of Traditional Chinese Medicine

Summary

This protocol describes a dependable and efficient in vitro model of the brain blood barrier. The method uses mouse cerebral vascular endothelial cells bEnd.3 and measures transmembrane electrical resistance.