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Neuroscienze
3D Bioprinted Cocultures of iPSC-Derived Neurons and Astrocytes

3D Bioprinted Cocultures of iPSC-Derived Neurons and Astrocytes

September 29, 2023

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1MSD Research Laboratories, London, UK, 2Merck & Co., Inc., Rahway, NJ, USA

Summary

Here, we present a protocol to produce 3D-bioprinted cocultures of iPSC-derived neurons and astrocytes. This coculture model, generated within a hydrogel scaffold in 96- or 384-well formats, demonstrates high post-print viability and neurite outgrowth within 7 days and shows the expression of maturity markers for both cell types.

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