An Optimized Mouse Embryonic Stem Cell Based Reverse Poly-Transfection Technique for Rapid Exploration of Nucleic Acid Ratios

Kieran Maheden; Karen Hwang; Ipek Egilmez; Nika Shakiba

doi:10.3791/65766

Rivista

Biologia dello sviluppo

Comparative Analysis of Forward and Reverse Transfection Methods on mPSCs

December 08, 2023

Author Spotlight: Advancements in Synthetic Genetic Devices for Stem Cell Fate Manipulation and Cellular Therapy Development

01:23 min

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Preparation of Mouse Pluripotent Stem Cells (mPSCs) for Transfection

01:21 min

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Reverse and Forward Transfection of Mouse Pluripotent Stem Cells (mPSCs)

01:35 min

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Flow Cytometry Analysis of Transfected Mouse Pluripotent Stem Cells (mPSCs)

01:50 min

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Kieran Maheden¹, Karen Hwang¹, Ipek Egilmez¹, Nika Shakiba¹

¹School of Biomedical Engineering,University of British Columbia

Summary

The present protocol describes a method for reverse poly-transfection of mouse embryonic stem cells during culture with 2i and LIF media. This method yields higher viability and efficiency than traditional forward transfection protocols, while also enabling one-pot optimization of plasmid ratios.