Sampling Cerebrospinal Fluid and Blood from Lateral Tail Vein in Rats During EEG Recordings

Marie Soukupov&#225;; Annunziata Guarino; Laila Asth; Pietro Marino; Mario Barbieri; Michele Simonato; Silvia Zucchini

doi:10.3791/65636

Rivista

Neuroscienze

Cerebrospinal Fluid and Blood Withdrawal from Rats with Concurrent EEG Recording

September 01, 2023

Author Spotlight: Obtaining High-Quality CSF and Blood Samples for Epilepsy Biomarker Discovery

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Tethered Video-EEG in Epileptic Rats for Analyses of Seizure Activity

02:11 min

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Collection of Blood from the Tail Vein of Rats During EEG Recordings

03:18 min

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Collection of Cerebrospinal Fluid from Rat Cisterna Magna During EEG Recordings

03:11 min

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Marie Soukupová¹, Annunziata Guarino¹, Laila Asth¹, Pietro Marino¹, Mario Barbieri¹, Michele Simonato¹, Silvia Zucchini¹

¹Department of Neuroscience and Rehabilitation, Section of Pharmacology,Universita degli Studi di Ferrara

Summary

The protocol shows repeated cerebrospinal fluid and blood collections from epileptic rats performed in parallel with continuous video-electroencephalogram (EEG) monitoring. These are instrumental for exploring possible links between changes in various body fluid molecules and seizure activity.