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Unveiling Mitochondrial Ultrastructure in Neurons Using STED Microscopy

Unveiling Mitochondrial Ultrastructure in Neurons Using STED Microscopy

June 30, 2023

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Propagation, Differentiation, and Preparation of SH-SY5Y Cells for Live Cell Imaging

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Imaging SH-SY5Y Cells by STED Microscopy and Subsequent Analysis of Mitochondrial Ultrastructure

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1Center for Open Research Resources and Equipment,University of Connecticut, 2Department of Molecular and Cell Biology,University of Connecticut

Summary

This protocol presents a workflow for the propagation, differentiation, and staining of cultured SH-SY5Y cells and primary rat hippocampal neurons for mitochondrial ultrastructure visualization and analysis using stimulated emission depletion (STED) microscopy.

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