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Imaging Live Brain Explants from Drosophila melanogaster Third Instar Larvae

Imaging Live Brain Explants from Drosophila melanogaster Third Instar Larvae

June 23, 2023

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

01:30 min
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1,086 Views

Staging and Collecting Drosophila melanogaster Third Instar Larvae

00:52 min
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243 Views

Fat Body Collection from Drosophila melanogaster Third Instar Larvae

00:55 min
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729 Views

Dissection of Drosophila melanogaster Third Instar Larval Brain for Live-Cell Imaging

01:41 min
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428 Views

Mounting and Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brain

02:38 min
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214 Views
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1Department of Biology,University of Washington

Summary

Here, we discuss a workflow to prepare, dissect, mount, and image live explant brains from Drosophila melanogaster third instar larvae to observe the cellular and subcellular dynamics under physiological conditions.

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