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Locus-Specific Chromatin Isolation from Yeast Cells

Locus-Specific Chromatin Isolation from Yeast Cells

November 17, 2023

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

01:51 min
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495 Views

Yeast Cell Culture and Cell Harvesting for Single-Copy Gene Locus Chromatin Purification

02:10 min
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130 Views

Locus-Specific Chromatin Purification from Saccharomyces cerevisiae

03:22 min
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64 Views

TEV Protease-Mediated Elution and Denaturation Elution of the Chromatin Locus Purified from Saccharomyces cerevisiae

03:17 min
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96 Views
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1Institute of Epigenetics and Stem Cells,Helmholtz Zentrum München

Summary

This protocol presents a locus-specific chromatin isolation method based on site-specific recombination to purify a single-copy gene locus of interest in its native chromatin context from budding yeast, Saccharomyces cerevisiae.

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