Pumpless Multi-Channel Flow Culture to Maintain and Assess Tissues and Cells

Pumpless Multi-Channel Flow Culture to Maintain and Assess Tissues and Cells

July 14, 2023

Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment

Setting Up the Pump-Free Multi-Channel Fluidic System and Loading the Tissue for Culturing

Matthew K. Grumbine¹, Varun Kamat², Khang Bao¹, Trevor Crupi¹, Kedar Mokate², Rayne Lim³, Jennifer R. Chao³, Brian M. Robbings⁴, Daniel T. Hass⁴, James B. Hurley⁴, Ian R. Sweet^1,2

¹EnTox Sciences, Inc, ²UW Medicine Diabetes Institute,University of Washington, ³Department of Ophthalmology,University of Washington, ⁴Department of Biochemistry,University of Washington

Summary

Real-time analysis of live tissue yields important functional and mechanistic data. This paper describes the protocols and critical variables to ensure accurate and reproducible generation of data by a novel and pump-free multi-channel fluidics system that maintains and assesses a wide range of tissue and cell models.

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