Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina

Shichu Chang; Wenjin Xu; Weijia Fan; John A. McDaniel; Marta Grannonico; David A. Miller; Mingna Liu; Hao F. Zhang; Xiaorong Liu

doi:10.3791/65237

Rivista

Neuroscienze

Mouse Retina OCT Fibergram Alignment with Confocal Images

June 30, 2023

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01:26 min

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In Vivo Visible Light Optical Coherence Tomography (OCT) Mouse Retinal Imaging

02:20 min

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Ex Vivo Confocal Imaging of Mouse Retina

02:34 min

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Alignment of In Vivo and Ex Vivo Mouse Retinal Images

01:25 min

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Shichu Chang¹, Wenjin Xu¹, Weijia Fan², John A. McDaniel¹, Marta Grannonico¹, David A. Miller², Mingna Liu¹, Hao F. Zhang², Xiaorong Liu^1,3,4,5

¹Department of Biology,University of Virginia, ²Department of Biomedical Engineering,Northwestern University, ³Department of Ophthalmology,University of Virginia, ⁴Program in Fundamental Neuroscience,University of Virginia, ⁵Department of Psychology,University of Virginia

Summary

The present protocol outlines the steps for aligning in vivo visible-light optical coherence tomography fibergraphy (vis-OCTF) images with ex vivo confocal images of the same mouse retina for the purpose of verifying the observed retinal ganglion cell axon bundle morphology in the in vivo images.