Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue&#45;Resident Macrophages <i>In Vivo</i> in Mice

Mark Gurney; Luke C. Davies; Ruth E. Jones; Valentina M. Bart; Robert H. Jenkins; Paul Brennan; Philip R. Taylor; Magdalena A. Czubala

doi:10.3791/64926

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Immunologia e infezioni

Lentiviral Vector Preparation for In Vivo Gene Modulation

February 16, 2024

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

00:55 min

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Transfection of HEK293T Cells Using Non-Liposomal Lipid Transfection Reagent for Lentiviral Production

02:06 min

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Sucrose Density Gradient Based Purification of Lentivirus Particles for Gene Delivery

03:41 min

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Mark Gurney¹, Luke C. Davies^1,2, Ruth E. Jones¹, Valentina M. Bart¹, Robert H. Jenkins¹, Paul Brennan¹, Philip R. Taylor^1,3, Magdalena A. Czubala¹

¹Systems Immunity Research Institute, Division of Infection and Immunity,Cardiff University, ²Biomedical Sciences Unit, Faculty of Medicine, Health and Life Science,Swansea University, ³UK Dementia Research Institute

Summary

We demonstrate a step-by-step protocol for the investigation of gene function in peritoneal tissue-resident macrophages in vivo, using lentiviral vectors.

Lentiviral Vector Preparation for In Vivo Gene Modulation

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

Transfection of HEK293T Cells Using Non-Liposomal Lipid Transfection Reagent for Lentiviral Production

Sucrose Density Gradient Based Purification of Lentivirus Particles for Gene Delivery

Summary

Article